Warriors Club Captain Ted Hill has sustained an ankle injury training with England.
Hill won his second cap as a replacement lock in last Sunday’s victory over USA at Twickenham and was expected to be involved in Saturday’s international against Canada.
The injury has ruled Hill out of the match. He will remain in camp with England while the medical staff await further assessments to establish the extent of his injury.
Bath Rugby lock Josh NcNally has also been ruled out of the Canada match after becoming the sixth England player to be injured in training.
“It has been a long season and injuries happen,” said England Forwards Coach Matt Proudfoot.
“It’s part of the game, we try to mitigate as much as we can, limit the amount of contact that we do.
“But you can get hurt stepping out of the shower. That’s just the way rugby is.
“I feel sorry for the guys that have worked so hard, both of them have worked incredibly, incredibly hard.”